Health Care Professionals
Mechanism of Action
- After instillation of Cysview® solution into the bladder, Cysview penetrates the cellular membrane of cancer cells.
- In these cells, Cysview is metabolized by enzymes in the heme biosynthetic pathway, thus leading to accumulation of protoporphyrin IX (PpIX). The immediate precursor to heme PpIX is a photoactive porphyrin (PAP) with fluorescence excitation between 360–450 nm (blue light) and emission peak at 635 nm (red or pink light).
- PAPs selectively accumulate in rapidly proliferating cells (e.g., tumor cells). After one hour, sufficient PAPs have been generated.
- Under blue light illumination (blue light cystoscopy), the PAPs emit pink light, facilitating specific and accurate visualization of the tumor.
Diagnosis with blue light cystoscopy relies on the selective accumulation of PpIX in neoplastic cells, with levels up to 10 times greater in tumors than in normal tissue following Cysview instillation. This leads to high fluorescence intensity and excellent contrast between normal and malignant urothelial cells.
Learn more about Blue Light Cystoscopy with Cysview® and the MOA pathway in the video below.